STUDIES ON TLR4 GENE POLYMORPHISM WITH EFERENCE TO PRODUCTION OF SERUM LYSOZYME AND IgG IN GOATS

Toll-like receptors (TLRs) are single transmembrane cell-surface receptors, which play a significant role in the innate immune system. Lysozyme is abundant in the body fluid and it is considered as a non-specific bactericidal substance. Serum immunoglobulin levels provide key information on the humoral immune status of the animals. The aim of the present investigation is to find out the polymorphism of TLR4 gene and the level of serum lysozyme and IgG in the three breeds of goats. PCR-RFLP of 494 bp amplicon of TLR4 gene shown AA and AB genotypes in Beetal goats, whereas Jamunapari and Black Bengal goats were found monomorphic and having only AA genotype. The allelic frequencies of A and B alleles in Beetal were 0.67 and 0.33 respectively, while the frequency of A allele in Jamunapari and Black Bengal was 1.0. Serum concentration of IgG in Beetal was significantly (P<0.01) higher by 5.52 mg/ml and 4.22 mg/ml than the Jamunapari and Black Bengal goats respectively. Beetal had higher concentration of serum lysozyme than the Janmunapari and Back Bengal. The study shows that Beetal goat breeds having high serum IgG and Lysozyme level may be selected for relatively better disease resistance against bacteria and virus.